Journal: Drug Design, Development and Therapy

Article Title: A Novel Octapeptide Derived From G Protein-Coupled Receptor 124 Improves Cognitive Function Via Pro-Angiogenesis In A Rat Model Of Chronic Cerebral Hypoperfusion-Induced Vascular Dementia

doi: 10.2147/DDDT.S226473

Figure Lengend Snippet: The GCPF peptide targets integrin αvβ3. Notes: ( A ) Ribbon drawing of the integrin αvβ3-GCPF structure simulated with molecular docking programs. In this figure, αv and β3 are shown in pink and green, respectively. The peptide is bound to the binding site (yellow). The carbon, nitrogen, and oxygen atoms of GCPF are shown in white, blue and red, respectively. The magnesium ions are shown as greenish blue balls. ( B ) Surface represents the GCPF-binding site. The GCPF peptide is shown as the stick model. The residues of the binding pocket in the structure of integrin αvβ3 are labeled. ( C ) The 2D image of interactions between GCPF and integrin αvβ3. ( D ) The dissociation constant of the GCPF peptide and integrin αvβ3 determined by ELISA experiments, where A 0 and A are the absorbance measured in the absence of the receptor and at a given concentration of the receptor, respectively. n=3. Mean ± SD.

Article Snippet: The dissociation constant (K d ) of GCPF and integrin αvβ3 interaction were determined in solution by ELISA as described by Friguet et al Briefly, integrin αvβ3 at indicated serial concentrations (0.5–32 nM) was incubated with the GCPF peptide (0.5 nM) for 4 hrs at 37°C to reach equilibrium; 150 μg of the mixture was transferred into the 96-well polystyrene plates that were precoated with integrin αvβ3 antibodies (dilution 1:50, SANTA CRUZ, CA) and incubated for 1 hr at room temperature.

Techniques: Binding Assay, Labeling, Enzyme-linked Immunosorbent Assay, Concentration Assay